Figure 6
From: Cloning, expression and characterization of gE protein of Duck plague virus
The transcription analysis of DPV gE in mock-infected and DPV-infected DEFs. A. Total RNA isolated from mock-infected and DPV-infected cells at various times post-infection was analysed using 1.0% agarose gel electrophoresis. B. The transcription analysis of DPV gE in infected DEFs with RT-PCR. The upper panel shows RNA from mock-infected DEFs (UN) and infected DEFs at different times (4, 5, 6, 8, 12, 16, 24, 36, 48, and 60 h) post-infection amplified by RT-PCR. M: molecular mass marker DL2000. The lower panel shows β-actin, which was run as an RNA-competence control. C. The transcription analysis of DPV gE in infected DEFs with. real time PCR. The DPV gE gene transcriptional expression was normalized to a reference gene (β-actin) and relative to the cells at 4 h post-infection. The average relative content of the DPV gE gene transcripts was calculated at 4, 5, 6, 8, 12, 16, 24, 36, 48, and 60 h post-infection using the 2-ΔΔCt method.