Figure 2
From: Cloning, expression and characterization of gE protein of Duck plague virus
The optimization analysis of the expression conditions of the pET32a/DPV-gE protein. A. the pET32a/DPV-gE protein was expressed in E. coli Rosseta, BL21(DE3), BL21(pLysS) host strains. M: Protein molecular weight marker; Lane 1, the pET32a/DPV-gE before induction in E. coli Rosseta; Lane 2, the pET32a/DPV-gE after induction in E. coli Rosseta (about 74 kDa); Lane 3, the pET-32a(+) after induction in E. coli Rosseta (about 27 kDa); Lane 4, the pET32a/DPV-gE before induction in E. coli BL21(DE3); Lane 5, the pET32a/DPV-gE after induction; Lane 6, the pET-32a(+) after induction; Lane 7, the pET32a/DPV-gE before induction in E. coli BL21(pLysS); Lane 8, the pET32a/DPV-gE after induction; Lane 9, the pET-32a(+) after induction; B. Effect of different temperature of the pET32a/DPV-gE protein in Rosseta. M: Protein marker; Lane 1, the pET32a/DPV-gE before induction in E. coli Rosseta; Lane 2-4, the pET32a/DPV-gE after induction at 25, 30, and 37°C; Lane 5, the pET-32a(+) after induction; C. Effect of different time of the pET32a/DPV-gE protein in Rosseta. M: Protein marker; Lane 1-6, the pET32a/DPV-gE protein was expressed respectively for 2, 3, 4, 4.5, 5, and 6 h after induction with 0.2 mM IPTG; Lane 7, the pET32a/DPV-gE before induction; Lane 8, pET-32a(+) after induction; D. Production of recombinant plasmid pET32a/DPV-gE from Rosseta in different IPTG concentrations. M: Protein marker; Lane 1-5, the pET32a/DPV-gE protein was expressed respectively after induction with 0.1, 0.2, 0.4, 0.8, and 1.0 mM IPTG; Lane 6, the pET32a/DPV-gE before induction; Lane 7, the pET-32a(+) after induction.