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Table 2 Percent BiFC positive cells in wells transfected with a combination of Vpr and Gag expression plasmids.

From: Human immunodeficiency virus type 1 Vpr: oligomerization is an essential feature for its incorporation into virus particles

Vpr plasmids

VC-Gag

VN-Gag

VC-Vprwt

0

31

VN-Vprwt

27

0

VC-VprA30L

0

0

VN-VprA30L

0

0

VC-VprΔ44

0

0

VN-VprΔ44

0

0

VC-VprE48A

0

29

VN-VprE48E

28

0

VC-VprL68E

0

0

VN-VprL68E

0

0

VC-VprH71R

0

0

VN-VprH71R

0

0

VC-VprR80A

0

33

VN-VprR80A

27

0

  1. 293T cells were cotransfected with combinations of VC and VN-chimeric Vpr and Gag expression plasmids as described in methods. Thirty-six hours post transfection cells were fixed and assessed by flow cytometry. BiFC positive (%) cells are presented in the respective combination. Results represent one of three independent experiments with similar results.