Skip to main content
Figure 7 | Virology Journal

Figure 7

From: Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone

Figure 7

The production of infectious HCV particles in the Huh-7.5 cells expressing NS3 antibody and control antibody. 1 × 106 cells were seeded in a 100-mm plate was infected with HCV-GFP virus at a MOI of 1.5. Cell free supernatants were collected every 96 hours. Three milliliter of culture supernatants was used to infect freshly cultured Huh-7.5 cells. The infectivity assay was repeated for four passages. The production of infectious HCV using RNA extracts from 1 ml of culture supernatants was determined by real-time RT-PCR. (black diamond) Huh-7.5 only, (black triangle) Huh-7.5 cells expressing NS3 antibody, (black square) Huh-7.5 cells expressing control antibody.

Back to article page