Skip to main content
Figure 6 | Virology Journal

Figure 6

From: Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone

Figure 6

Infectivity assay of HCV-GFP chimera virus in Huh-7.5 cells expressing NS3 antibody and control antibody. Huh-7.5 cells (1 × 104) were seeded on a 2-well glass chamber slide, next day infected with JFH1-GFP virus for overnight at 37°C. Infected cells were examined for GFP expression after 24, 48, 72 and 96 hours of post infection. Superimposing blue (nucleus) with green fluorescence (HCV GFP) using the Abode Photoshop computer software generated the final image. Upper panel: shows HCV-GFP expression in Huh-7.5 cells infected with JFH1 virus. Middle panel: shows HCV-GFP expression was terminated in Huh-7.5 cells expressing NS3 antibody. Lower panel: shows no reduction in the level of HCV-GFP expression in Huh-7.5 cells expressing control antibody.

Back to article page

Virology Journal

ISSN: 1743-422X