A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control

Table 5 Primers/probes used in the study

Primer/probe	Primer/probe sequence (5'--3')	Position
As	5'-GAGTAGTGTTGGGTCGCGAA-3'	256--275
Aa	5'-GTGCACGGTCTACGAGACCTC-3'	320--340
Ap	FAM5'-CCTGATAGGGTGCTTGCGAGTGCC-3' BHQ	292--315
Bs	5'-AGCGTCTAGCCATGGCGTTAGTAT-3'	74--97
Ba	5'-TCCTCGCAATTCCGGTGTACTC-3'	161--182
Bp	FAM5'-CCCCCCTCCCGGGAGAGCCATAGT-3' BHQ	121--144
ICp	Cy55'-TTCCGCTGCCTGCTCAGTCGATCC-3' BHQ

ISSN: 1743-422X