A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control

Table 1 Optimization of the concentration of IC

Armored RNA concentration (copies/ml)	National reference material 4 for HCV RNA 8.5 × 10⁵ IU/ml		National reference material 3 for HCV RNA 3.97 × 10⁴ IU/ml		National reference material 2 for HCV RNA 2.26 × 10³ IU/ml		National reference material 1 for HCV RNA 2.26 × 10² IU/ml		HCV 0 IU/ml
	IC (Cy5)Ct	HCV (FAM)Ct	IC (Cy5)Ct	HCV (FAM)Ct	IC (Cy5)Ct	HCV (FAM)Ct	IC (Cy5)Ct	HCV (FAM)Ct	IC (Cy5)Ct
100000	31.92	27.00	31.43	32.77	30.98	>45	30.43	>45	30.13
10000	36.51	26.93	35.39	31.20	34.58	36.29	34.29	41.55	34.21
1000	>45	26.44	38.35	30.14	37.72	33.23	36.68	36.67	36.47
0	>45	26.21	>45	30.11	>45	32.14	>45	36.12	>45

Concentrations of HCV RNA and armored RNA were indicated by FAM and Cy5 signals, respectively