Figure 4From: A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal controlConstruction of IC by using overlap extension PCR. (a) The internal probe-binding sequences were introduced into the HCV 5' UTR sequence by 3 cycles of PCR using primers designed by amplifying overlapping regions. (b) Constructed IC sequence. The blue portion represents the internal probe-binding sites.Back to article page