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Figure 3 | Virology Journal

Figure 3

From: A novel duplex real-time reverse transcriptase-polymerase chain reaction assay for the detection of hepatitis C viral RNA with armored RNA as internal control

Figure 3

Principles of the duplex real-time RT-PCR for detection of HCV RNA. The assay was performed using 2 sets of primers/probes and a specific armored RNA as IC. Both the primer/probe sets A and B, in combination, detected the HCV 5' UTR sequence. Further, both the detection probes were labelled with the same fluorophore, i.e. FAM, at the 5' end and with the same quencher dye, i.e. Black Hole Quencher (BHQ), at the 3' end. The ICs had the same primer-/probe-binding sites and amplification efficiencies as the target nucleic acid but contained discriminating probe sequences.

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