Figure 2From: Use of monoclonal antibodies against Hendra and Nipah viruses in an antigen capture ELISAEpitope mapping of mAb 1A11 C1 using direct ELISA. Synthetic peptides corresponding to the complete NiV N protein sequence (a.a. 1-532) were serial diluted and coated at concentration from 1 μg to 0.1 ng per well (100 μL in volume). A peptide from Alkhurma virus E protein (Alk E, a.a. 143-168) was included as negative control and signal cutoff value (0.17) was calculated based on readings from this peptide. NiV infected Vero lysate diluted 10 fold to 105 fold were served as positive control.Back to article page