Figure 1

Generation of recombinant RSV-X virus. A) Schematic diagram of the RSV-X genome (genome length 15213 nt) and positions of the genetic tags inserted in the cDNA copy of the rRSV-X and ΔG constructs. The SexA I, Xma I, BssH II, Bsiw I, and Mlu I sites were introduced to facilitate construction. ΔG was recovered by excision of the fragment BssH II and BsiW I and subsequent religation of the vector. B) Expression of RSV proteins by rRSV and ΔG deletion recombinant viruses. Vero cells were infected at an m.o.i of 0.1 TCID₅₀/ml. At 72 hr post infection cell monolayers was harvested and subjected to Western blotting using antiserum against RSV. The molecular weight size markers are depicted on the left and the position of the major RSV proteins are indicated at the right [29].