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Table 2 Biochemical and topological attributes of CDV-R MPV mutations

From: Comparative whole genome sequence analysis of wild-type and cidofovir-resistant monkeypoxvirus

Protein

ORFa

Amino Acid

Domain

Δ Polarityb

Δ Hydropathyb

Surface Exposured

Surface Contoure

DNA pol

E9L

A314V

exonuclease

0.1

2.4

decrease

increase

DNA pol

E9L

A613T

polymerase

6.8

2.5

increase

increase

DNA pol

E9L

A684T

polymerase

6.8

2.5

increase

increase

DNA pol

E9L

T808M

NA

6.4

2.6

decrease

decrease

RNA pol subunit rpo147

J6R

K355N

TFIIB docking

5.3

3.12

decrease

increase

RNA pol subunit rpo147

J6R

L653R

funnel

22.3

8.3

increase

increase

mRNA capping enzyme small subunit

D12L

H122Y

dimerization

4.2

1.9

increase

decrease

mRNA capping enzyme small subunit

D12L

S186N

dimerization

4.6

2.7

increase

decrease

poly-A pol catalytic subunit VP55

E1L

R342H

dimerization

9.7

0.7

decrease

decrease

  1. aspecifies ORFs relative to Copenhagen strain. bchanges in polarity and hydropathy due to amino acid substitutions were calculated using Kyle and Doolittle algorithm in Lazergene (DNAstar) software. dsurface exposure and econtour were determined using the Emini method and Jameson-Wolf algorithm, respectively (DNAstar software).