Figure 3

Multiplication of rVACV and sFL production in macrophage cell line J774.G8. Culture of J774.G8 cells was infected with the purified virus at MOI of 2.5 (A, C, D) or at MOI of 0.1 (B) at 37°C for 1 hour, washed with PBS and the fresh medium with or without cytosine arabinoside (40 μg/ml) was added. Multiplication of virus was determined as beta-galactosidase activity (A, C) or by Q-PCR (B) at indicated intervals (A, B) or 24 hours after infection (C). Total FL production was determined by ELISA (D).