Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis

Table 1 Different expression of proteins between H-PRRSV (H96, H168) inoculated lungs and control identified by MALDI-TOF or MALDI-TOF/TOF.

Master no.^a	Accession no.^b	Human protein (Abbr.)	p Value^c	Mr (Da)	pI	Protein score^d	Sequence Coverage (%)^e
187	gi\|134085736	ARHGAP29	0.0044	107466	5.65	69	13
342	gi\|136192	TF	0.0047	78971	6.93	71	15
352	gi\|74005206	NDUFS1	0.0045	81056	6.1	71	15
371	gi\|126309857	HSPA8	0.0055	54213	5.74	97	25
381	gi\|194037328	KRT79	0.0087	48266	6.07	67	18
467	gi\|68317041	STIP1	0.0029	32224	8.86	106	58
507	gi\|231467	AHSG	0.0036	39199	5.5	73	10
552	gi\|82822840	DPYSL2	0.0018	31025	8.07	84	11
690	gi\|2724046	ACTG1	0.00037	36099	5.65	112	10
882	gi\|27807289	ANXA2	0.0089	38873	6.92	73	6
885	gi\|23706161	IDH3A	0.0014	27981	9.62	165	15
1461	gi\|809283 + gi\|1709082	HBB	0.0031	16082 + 19200	6.76 + 6.37	102 + 70	60 + 43

a) Master no. is the unique sample spot protein number.
b) Accession is the MASCOT result of MALDI-TOF/TOF searched from the NCBI nr database.
c) The p value of ONE-ANOVA, p < 0.01, or Independent Student's t-test contrast, p < 0.05.
d) Protein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification.
e) Sequence coverage (%) is the number of amino acids spanned by the assigned peptides divided by the sequence length.

ISSN: 1743-422X