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Table 2 Epitope-specificity assay of monoclonal antibody by ELISA*

From: Monoclonal antibody induced with inactived EV71-Hn2 virus protects mice against lethal EV71-Hn2 virus infection

Antibody

Expressed VP1

Hn2 virus

PBS

Peptide

4D10

0.400

2.551

0.056

-

3B11

0.338

1.740

0.072

-

4E8

1.820

2.665

0.034

1.374 (P25:aa240-250)

1.448 (P26:aa250-260)

4C6

1.727

2.774

0.080

0.705 (P25:aa240-250)

    

0.488 (P26:aa250-260)

Normal mouse serum

0.113

0.611

0.045

0.072

  1. *The 96-well microtiter plates were coated with 500 ng of purified EV71 Hn2 virus, E.coli expressed VP1 protein or synthetic VP1 peptide P25 or P26. Monoclonal antibodies were at a dilution of 1:2000. OD450 nm readings are expressed as the average of of three separate experiments.
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Virology Journal

ISSN: 1743-422X