Figure 3
From: The novel HSV-1 US5-1 RNA is transcribed off a domain encoding US 5, US 4, US 3, US 2 and α22
The U S 5-1 RNA spans genomic region between U S 5 and ICP22. Cultures of RSC in 25 cm2 flasks were infected with 10 PFU of HSV-1(F) and total RNA was extracted 18 h after infection. The 3'- and 5'-ends were determined by RNase protection assay. In addition to samples from infected cells (panel A, lane 3 and panel B, lane 4), included were control samples of the equivalent amount of yeast total RNA, untreated (panel A, lane 2 and panel B, lane 2) or treated with RNAseA/RNaseT1 mix (panel A, lane 4 and panel B, lane 3). Single-stranded RNA marker was included for size determination (panels A and B, lane 1).