Figure 1
From: The novel HSV-1 US5-1 RNA is transcribed off a domain encoding US 5, US 4, US 3, US 2 and α22
Genomic location of the U S 5-1 RNA. (A) In red is shown the sequence of the probe 3, used for the determination of the 5'-end of the US5-1 RNA. Underlined are first 270 bases of the probe 3, which was the size of the protected fragment in the RNase protection experiment to determine the 5'-end of the US5-1 RNA. In the box is the sequence of potential TATA box. (B) In red is shown the sequence of the probe 1, used for the determination of the 3'-end of the US5-1 RNA. Underlined are the last 180 bases of the probe 1, which was the size of the protected fragment. Sequence of a possible polyadenylation signal for the US5-1 transcript is shown in the box. (C) Location and the direction of transcription of RNAs expressed from the unique-short region are represented by arrows. Red arrows represent protein-coding RNAs, black arrows represent non-coding RNAs. Location and the direction of the transcription of the single-stranded RNA probes used in the experiments described in this report are represented by green arrows. Primers used for RT-PCR detection of US5-1 transcript are shown in blue arrowheads.