Figure 5
Therapeutic potential of 5'PPP-RNA. A459 cells (1 × 106 cells/well) in a 6-well tissue culture plate were infected with (i) wild-type A/New York/02/2001 and its (ii) H274Y oseltamivir-resistant A/New York/02/2001 variant for the 0 hr, 4 hr or 8 hr before treatment with 5'PPP-RNA or CIAP-RNA. Supernatants collected after 24 hr of infection were assayed for viral titers as indicated in material and methods. Cells were harvested to assay IFNβ (iii) and RIG-I (iv) mRNA level by Real time RT-PCR. Results shown are mean ± SD from three independent experiments and are expressed as viral titer (pfu/ml) or fold increase over controls.