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Figure 1 | Virology Journal

Figure 1

From: 5'PPP-RNA induced RIG-I activation inhibits drug-resistant avian H5N1 as well as 1918 and 2009 pandemic influenza virus replication

Figure 1

5'PPP-RNA suprresses the replication 1918 virus as well as wild-type and drug-resistant H5N1 viruses. (A) A549 (1 × 106 cells/well) in a 6-well tissue culture plate were mock-transfected (control) or transfected with 2 μg of 5'PPP-RNA or CIAP-RNA using lipofectamine 2000 and 24 hr later were infected with (i) 1918 pandemic virus (0.01 MOI). (ii), Transfected A549 cells were also infected with wild-type H5N1 (0.1 MOI) following 0, 24 and 48 hr of transfection. B. Mock, 5'PPP-RNA or CIAP-RNA transfected A549 cells were infected with drug-resistant H5N1 viruses A/VN/1203/2004 (i), A/VN/30408/2004 H274Y(ii) and A/VN/30408/2004 N294S (iii) 24 hr post transfection. Supernatants collected after 24 hr of infection were assayed for viral titers and results shown are mean ± SD of three independent experiments and are expressed as viral titer (pfu/ml).

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