Figure 2

Antibody conjugate stability analysis. The antibody conjugates were diluted 1:20 with phosphate buffered saline (PBS) (0.01 mol/L, pH 7.4) containing 1% bovine serum albumin (BSA) and 10 mM histidine. After incubation at 20°C, 4°C, 37°C, and room temperature for 0, 1, 2, 4, and 8 weeks, samples were removed at each time point and analyzed by enzyme-linked immunosorbent assay (ELISA) using a HEV IgM Diagnostic Kit. The results showed that the antibody conjugate was stable enough to be used in external quality assessment and internal quality control trials.