Figure 2

Diverse phenotypes of protoplast regenarants of S. sclerotiorum hypovirulent strain XG36-1. A, three types of colony morphologies, namely Type I, Type IIand Type III, were developed on PDA plate at 20–22 C for 15 days. Regenarants in Type Iwere not significantly different from wildtype strain XG-13, while regenarants in Type III were similar to strain XG36-1 (see Figure 1), Type II were intermediate type between Type Iand Type III. B and C, comparison of the hyphal growth rate on PDA plate and virulence on detached rapeseed leaves among three types of regenarants. The growth and virulence of regenarants in TypeI were fully recovered, and that in Type II were partially recovered; the growth and virulence of regenarants in Type III was not significantly different from strain XG36-1.