Table 1 Compared primer-probe sets
From: Improvement of a real-time RT-PCR assay for the detection of enterovirus RNA
| Â | Primer-probe set 1 (earlier reported) | Primer-probe set 2 |
|---|---|---|
Forward primer | 5'-CCCTGAATGCGGCTAATCC-3' Genbank: NC_002058.3 (452–470) | 5' CCGGCCCCTGAATGC-3' Genbank: NC_002058.3 (447–461) |
Reverse primer | 5'-ATTGTCACCATAAGCAGCCA-3' Genbank: NC_002058.3 (596–577) | 5' CACCGGATGGCCAATCCA-3' Genbank: NC_002058.3 (639–622) |
Probe | FAM 5' AACCGACTACTTTGGGTGTCCGTGTTTC-3' TAMRA Genbank: NC_002058.3 (535–562) | |