Sensitivity of the PGMY09/11 (1A), MY09/11 (1B), and HPV-32 Specific (1C) PCRs were tested using dilutions of plasmid (pMJ32L1) containing HPV-32 L1 gene. Each assay was tested on a dilutions scheme starting at 500,000 down to 1 copy. Figure contains a representative gel from 6 experiments (three separate dilutions done in duplicate). The sensitivity of both the PGMY09/11 and MY09/11 PCR assays were shown to be approximately 3,000 copies of HPV-32 L1. The HPV-32 Specific PCR consistently detected 5 copies of HPV-32 L1.