Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir

Table 1 UL97 recombinant sensitivity to Maribavir

Virus	AA Mutation	Rb-binding Motif	MBV^a	CDV	GCV
HB5	wt	wt	0.37 ± 0.03	0.15 ± 0.05	3.6 ± 3
RC314	K355M	wt	14 ± 5	0.1 ± 0.07	38 ± 10
RC295	C151G	LxCxE	0.22 ± 0.08^c	0.18 ± 0.04	2.8 ± 0.9
RC312	C428G	LxCxD	0.39 ± 0.2	0.22 ± 0.1	4.2 ± 2
RC316	C693G	IxCxE	0.3 ± 0.1	0.18 ± 0.1	3.2 ± 3
RC323	C428G/C151G	LxCxD/LxCxE	0.5 ± 0.2	0.08 ± 0.05	3.5 ± 3
T2211^b	wt	wt	0.100 ± 0.008	0.23 ± 0.01	1.16 ± 0.18
T3038^b	ΔC151	LxCxE	0.031 ± 0.002^c	0.27 ± 0.01	1.03 ± 0.13

a. Values shown are the concentrations of drugs (μM) that are sufficient to reduce viral replication by 50% (EC₅₀) with standard deviation shown. Each value is the average of at least 3 experiments.
b Values were obtained using a surrogate assay from viruses containing a SEAP expression cassette at US6.
c. Values were reduced significantly from the isogenic control viruses as determined by the Student's T-test (p < 0.05).

ISSN: 1743-422X