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Table 1 UL97 recombinant sensitivity to Maribavir

From: Conserved retinoblastoma protein-binding motif in human cytomegalovirus UL97 kinase minimally impacts viral replication but affects susceptibility to maribavir

Virus AA Mutation Rb-binding Motif MBVa CDV GCV
HB5 wt wt 0.37 ± 0.03 0.15 ± 0.05 3.6 ± 3
RC314 K355M wt 14 ± 5 0.1 ± 0.07 38 ± 10
RC295 C151G LxCxE 0.22 ± 0.08c 0.18 ± 0.04 2.8 ± 0.9
RC312 C428G LxCxD 0.39 ± 0.2 0.22 ± 0.1 4.2 ± 2
RC316 C693G IxCxE 0.3 ± 0.1 0.18 ± 0.1 3.2 ± 3
RC323 C428G/C151G LxCxD/LxCxE 0.5 ± 0.2 0.08 ± 0.05 3.5 ± 3
T2211b wt wt 0.100 ± 0.008 0.23 ± 0.01 1.16 ± 0.18
T3038b ΔC151 LxCxE 0.031 ± 0.002c 0.27 ± 0.01 1.03 ± 0.13
  1. a. Values shown are the concentrations of drugs (μM) that are sufficient to reduce viral replication by 50% (EC50) with standard deviation shown. Each value is the average of at least 3 experiments.
  2. b Values were obtained using a surrogate assay from viruses containing a SEAP expression cassette at US6.
  3. c. Values were reduced significantly from the isogenic control viruses as determined by the Student's T-test (p < 0.05).