Skip to main content
Figure 4 | Virology Journal

Figure 4

From: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

Figure 4

HIV-1 subtype C Gag-specific serum IgG responses in mice vaccinated with recombinant Salmonella vaccine vector. Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Serum (pooled from 5 mice per group) was isolated from blood taken before each vaccination on day 0, 28 and 56 and just before sacrifice on day 84. (A) The HIV-1 Gag-specific IgG for each group of mice with a 1/100 serum dilution. The data are the ratio of the OD405 nm for vaccinated mice and the OD405 nm for the day 0 serum (pre-bleed). (B) The HIV-1 Gag-specific IgG1 and IgG2a were measured in serum of each group of mice on Day 84 with a 1/100 serum dilution. Each bar represents the mean OD405 nm value. Differences in antibody responses between vaccine groups at different time points were analyzed by a two-sample student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

Back to article page