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Figure 3 | Virology Journal

Figure 3

From: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

Figure 3

HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by the amount of cytokines secreted by stimulated cells. Groups of mice (5 per group) were vaccinated with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or antigen-negative Salmonella control vaccine (aroC+pGEM) as indicated in Figure 2. Splenocytes isolated and pooled from 5 mice per group on day 84 were incubated in R10 medium only (negative assay control), or stimulated with a Gag CD4 peptide for 48 hrs. Cytokines released into the supernatant were quantified using a mouse Th1/Th2 cytokine bead array (CBA) assay. (A) IFN-γ, (B) TNF-α, (C) IL-4 and (D) IL-5. Each bar in the graphs represents the average picogram amount of cytokine produced per 10e6 splenocytes in 48 hours of stimulation with media or Gag CD4 peptide, for triplicate responses. A p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

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Virology Journal

ISSN: 1743-422X