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Figure 2 | Virology Journal

Figure 2

From: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

Figure 2

HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell responses as evaluated by IFN-γ and IL-4 ELISPOT assays. Groups of mice (5 mice per group) were vaccinated three times (day 0, 28 and 56) with live recombinant Salmonella vaccine that expressed HIV-1 Subtype C Gag (aroC+Gag) or an antigen-negative Salmonella control vaccine (aroC+pGEM). Mice were sacrificed on day 84, and splenocytes were prepared from isolated spleens pooled from 5 mice per group then used in IFN-γ (A) and IL-4 (B) ELISPOT assays with R10 medium only (negative assay control) or with the Gag CD4 peptide. Bars are the mean number of spots from triplicate wells and are expressed as SFU/10e6 splenocytes. Differences in immune responses between vaccine groups were analyzed by a two-sample student's t-test. A p < 0.05 was considered statistically significant. The differences in response between stimulated cells and unstimulated cells within the same vaccine group were also analyzed by a student's t-test and a p < 0.05 was considered statistically significant. A p > 0.05 was considered not statistically significant (NS).

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Virology Journal

ISSN: 1743-422X