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Figure 1 | Virology Journal

Figure 1

From: Oral vaccination with a recombinant Salmonella vaccine vector provokes systemic HIV-1 subtype C Gag-specific CD4+ Th1 and Th2 cell immune responses in mice

Figure 1

The HIV-1 subtype C Gag expression plasmid (pGEM+Gag) and Gag expression by Salmonella. (A) The HIV Gag expression cassette contained the gag gene fused in-frame with the β-galactosidase α-gene and expression was under the E. coli lac (lactose) promoter. The plasmid contained an E. coli origin of replication (ori) and ampicillin resistance gene (AmpR). (B) The relative expression of the Gag by the recombinant Salmonella vaccine (aroC+Gag) was determined by SDS-PAGE and (C) the Roche Elecsys® HIV p24 Ag assay. In the Roche Elecsys® HIV p24 Ag assay, total bacterial protein lysate was diluted 1/1000 in water and the cut-off index was calculated by the Elecsys® 2010 analyzer using readings from the negative and positive calibrators.

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