Figure 1
Expression of recombinant HCV proteins. A. Individual HCV genes were cloned into pcDNA3.1(+) plasmid under CMV promoter. The expression of HCV proteins was verified by in vitro translation. The proteins were metabolically labeled with [35S]-methionine and separated on 15% (core, E1, NS4A) or on 12% (E2, NS2, NS3, NS5A, NS5B, NS4B) SDS-PAGE and autoradiographed. B. SDS-PAGE analysis of purified recombinant HCV proteins. Individual HCV genes were inserted into baculovirus expression plasmids and recombinant HCV-expressing baculoviruses were obtained. Recombinant HCV proteins were produced in Sf9 cells followed by purification of the proteins by preparative SDS-PAGE. Samples of purified HCV proteins (0.5–1 μg each) were separated on 15% (core, E1 and NS4A) or 12% (E2, NS3, NS5A, NS5B, NS2 and NS4B) SDS-PAGE and stained by Coomassie Blue.