Fractionation segregates anti-viral constituents. ED cells were infected with EIAVWSU5 in the presence of the size exclusion fractions (100 μg/mL) (A), ethanol-soluble sub-fractions (B), ethanol-insoluble sub-fractions (C), or a combination of both the ethanol soluble and insoluble fractions at concentrations that reconstitute the original fractions (D). Forty h following infection, cells were fixed and immunostained for viral antigen. Cell viability studies were performed in parallel. Cell viability and virus infectivity are shown as a ratio of the values in the presence of the fractions divided by the control. Data represent the average and standard error of three experiments performed in triplicate. *, p < 0.05; **, p < 0.001.