Prunella extracts do not destroy EIAV particles. EIAVWSU5 virions were incubated with DMSO, Triton X-100 (0.5%), or aqueous extracts of Prunella extract (0.4% final concentration, 132 μg/mL of Ames 27664 or 126 μg/mL of Ames 27748) for 1 h at 37°C. The treated virions were density banded on a 20–60% sucrose gradient. Eleven fractions were collected and immunoblotted for EIAV Gag proteins, Capsid (C) and Matrix (M). The experiment was repeated three times and a representative blot is shown.