Figure 5
From: Inhibition of lentivirus replication by aqueous extracts of Prunella vulgaris
Prunella extracts inhibit EIAV infectivity primarily by acting on viral particles. A) Ability of cell-associated Prunella extracts to inhibit EIAV infection. Extracts were incubated with ED cells and removed prior to the addition of EIAV. EIAV infection was evaluated at 40 h following infection. B) Ability of Prunella extracts to inhibit EIAV. 2.2 x 105 EIAVWSU5 particles were incubated with 25, 50 or 100 μg/mL extract from Prunella Ames 27664 extract for 10 minutes at room temperature. Viral stocks were diluted in media containing the appropriate concentration of extract and plated onto ED cells. Forty h following infection the cells were fixed, immunostained for EIAV antigen and enumerated. Shown are the numbers of infectious virions/ml. Data represent the average and standard error of three experiments performed in duplicate (A) or triplicate (B). Extracts significantly reduced EIAV virion infectivity at all concentrations tested.