Analysis of Ebola GP mutants in trans enhancement process. (A) HEK Tet-On cells expressing Wt EGP were challenged with EGP mutant viruses, GP1 mutants, relative infectivity of Wt and GP1 mutant viruses. Fusion peptide mutants, relative infectivity of Wt and fusion peptide mutant viruses. Coiled-coil mutants, relative infectivity of Wt and GP2 mutant viruses. (B) Matrix analysis of trans enhancement by EGP. Wt or mutant EGP expressing HEK Tet-On was either uninduced or induced with 1 μg/ml of Dox. After 24 h, the cells challenged with pseudovirus particles carrying Wt or mutant EGP. The luciferase activities in infected cells are represented as relative percentage of luciferase activity in uninduced cells. Data represents an average of three independent experiments. For clarity of the chart we have omitted the error bars in panel B.