Skip to main content

Table 3 P1: HAt/NA interactions in the yeast two-hybrid system

From: Identification and characterisation of a novel anti-viral peptide against avian influenza virus H9N2

DBD Vectorsa

AD Vectorsa

β-gal activityb

Background

  

BD

AD

0.05

BD-P1

AD

0.07

BD

AD-HAt

0.05

BD

AD-NA

0.03

P1:HA t /NA interactions

  

BD-P1

AD-HAt

25

BD-P1

AD-NA

3.5

  1. apHyblex/Zeo and pYESTryp2 are vectors encoding the LexA DNA binding domain (BD) and B42 transcriptional activation domain (AD), respectively.
  2. bAverage β-gal activity (Miller Units) of 3 independent colonies for each co-transformation (The SD value is not shown as there was very little variation between repeated experiment).