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Figure 4 | Virology Journal

Figure 4

From: Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

Figure 4

Northern hybridization analysis of DENV-2 replication in cells expressing hRz constructs. (A) Total RNA samples hybridized with DENV-specific probes. (B) Actin RNA from the same samples hybridized with a β-actin-specific probe. Each construct is indicated by numbers; i: wild-type C6/36 infected with DENV; u: uninfected wild-type C6/36. The autoradiograph was exposed for 6 hr prior to developing. (C) Quantification of relative DENV-2 RNA levels from the Northern blot analysis. The scanned autoradiograph was processed in ImageJ software and the relative amount of DENV-2-specific RNA in each sample was compared against that of infected wild-type cells using an ANOVA test (GraphPad Prism 3.0). Statistically significant differences relative to the Infection control (Dunnett's post-test, p < 0.01) are indicated with asterisks. Infected: infected, non-transduced C6/36 cells; Uninfected: uninfected, non-transduced C6/36 cells; Rz #: Different infected hRz cells.

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Virology Journal

ISSN: 1743-422X