Skip to main content
Figure 2 | Virology Journal

Figure 2

From: Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

Figure 2

RT-PCR of total RNA extracted from hRz-C6/36 cells. (A) hRz expression in the cells was detected by the presence of an hRz-specific band at about 100 bp. Primers for each hRz-C6/36 cells were specific to the hRz insert except for the control lacking a hRz sequence (-Rz) for which the primers were specific to tRNAval and poly(A) tail. (B) Wild-type C6/36 cells failed to give a PCR product when tested with 15 sets of primers (each primer was specific to each hRz). 1–14: 14 different hRz-C6/36 cells; -Rz: C6/36 cells without the hRz insert; wt: wild-type C6/36 cells; CtrlP: plasmid DNA control for PCR amplification; M: 1 Kb Plus DNA ladder; +RT: reactions with reverse transcriptase; -RT: reactions without reverse transcriptase.

Back to article page

Virology Journal

ISSN: 1743-422X