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Table 3 Expression of β-galactosidase in cells expressing UL82 homologs1

From: Properties of virion transactivator proteins encoded by primate cytomegaloviruses

Virus

Protein expressed

β-galactosidase activity

  

U373

HFFF2

in 1310

YFPpp71

181 (20)

240 (21)

in 0150

YFPS82

400 (20)

450 (73)

in 0145

YFPB82

497 (0)

521 (117)

in 0144

YFPRh82

353 (15

382 (94)

in 0146

YFPCh82

210 (10)

243 (16)

in 1374

None

0 (13)

35 (2)

  1. 1. Monolayers of U373 cells or HFFF2 cells were infected with 1 pfu/cell (U373) or 5 pfu/cell (HFFF2) of in 1374-based recombinants that expressed YFP-tagged UL82 homologs and maintained at 38.5°C for 10 h. At this time, extracts were prepared and assayed for β-galactosidase activity. Extracts were subsequently analyzed for protein production, as shown in figure 2. Background values (from mock infected cultures) were subtracted. The data presented here and in figure 2 are from the same lysates of a single experiment, and are representative of three independent experiments. The β-galactosidase activity from mock infected cells was subtracted from all values. The value in brackets is the maximum deviation from the mean of duplicate (U373) or triplicate (HFFF2) determinations.