Properties of virion transactivator proteins encoded by primate cytomegaloviruses

Table 2 Stimulation of gene expression by pp71 and S82¹

Cells	Preinfection	Stimulation of β-galactosidase expression²
		in 1382	in 1357	in 1383
HFFF2	in 1372	0.7	0.9	ND
HFFF2	in 1316	4.8	2.7	ND
HFFF2	in 1305	4.8	3.3	ND
Vero	in 1372	0.6	ND	ND
Vero	in 1316	5.1	4.7	ND
Vero	in 1305	3.7	4.0	ND
3T3	in 1316	12.6	7.3	5.6
3T3	in 1305	10.8	6.6	4.3

1. Monolayers were mock infected or infected with in 1372 (a control virus that expresses Cre recombinase), in 1316 (expresses pp71) or in 1305 (expresses S82) at moi 2, incubated at 38.5°C for 3 h, and infected with a second reporter virus (moi 0.5) that contained the lacZ coding region controled by the HCMV MIEP (in 1382), the SCMV MIEP (in 1357) or the HSV-1 ICP0 IE promoter (in 1383). After incubation for a further 5 h at 38.5°C, cell extracts were analysed for β-galactosidase activity.
2. The stimulation of β-galactosidase expression compared with the value for extracts of cells mock infected prior to infection with the reporter virus. Backgound activity from cells mock infected throughout was subtracted from all values. The means from at least two independent determinations are presented.

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