Figure 3
From: Properties of virion transactivator proteins encoded by primate cytomegaloviruses
Long term expression of β-galactosidase. Monolayers of HFFF2 cells were infected with in 1374-based recombinants (moi 3) and incubated at 38.5°C for 10 days in medium containing 2% fetal calf serum. Monolayers were stained for β-galactosidase activity on day 10 (top row), after superinfection with ts K on day 10 for 16 h (middle row), or after downshift to 32°C on day 10 and futher incubation for 4 days in medium containing 2% human serum (bottom row).