Figure 2
From: Properties of virion transactivator proteins encoded by primate cytomegaloviruses
Protein expression by in 1312-based recombinants. Monolayers of U373 cells were infected with 1 pfu/cell of in 1374-based recombinants that expressed YFP-tagged UL82 homologs and maintained at 38.5°C for 10 h. At this time, extracts were analysed for protein levels, using anti-GFP or anti-actin antibodies as probes. Monolayers were infected with in 1374 (lane 1), in 1310 (lane 2), in 0150 (lane 3), in 0145 (lane 4), in 0144 (lane 5), in 0146 (lane 6) or mock infected (lane 7). Extracts were also analysed for β-galactosidase activity, as described in table 1.