Figure 5
From: Biochemical typing of pathological prion protein in aging cattle with BSE
Biochemical typing of different brain regions in H-type BSE. Western blot analysis of the H-type BSE zebu (Charly-04) with a) a core-binding antibody (Sha31), b) an amino-terminal binding antibody (12B2) and c) a carboxy-terminal binding antibody (SAF84). Samples are assigned to the lanes as follows: negative control (N), L-type BSE (L), C-type BSE (C) and for the zebu medulla oblongata (lane 1, 15 mg tissue equivalent), cerebellar cortex (lane 2, 15 mg), hippocampus (lane 4, 0.75 mg), piriform lobe (lane 5, 15 mg), basal ganglia (lane 7, 1.5 mg), frontal cortex (lane 8, 15 mg), occipital cortex (lane 9, 15 mg) and temporal cortex (lane 10, 15 mg). The dashed line indicates the molecular mass of the unglycosylated C-type PrPres and helps to visualize differences compared to the H-type BSE zebu. The same samples, but deglycosylated are shown in d) with a carboxy-terminal binding antibody (SAF84). A molecular mass marker (in kDa) is indicated on the left.