Figure 5
From: Hepatitis C virus NS4B carboxy terminal domain is a membrane binding domain
Effect of NS4B carboxy terminal mutations on colony formation using selectable replicons. Colony formation assay in which Huh7 cells are transfected with in vitro transcribed replicon RNA that contain NS4B-CTD mutations. Colonies were stained using Coomassie blue. Wild-type is pFK5.1. Mock transfected cells as the control. The NS4B-CTD mutations TripleE, E247, E248 and helix-swop in pFK5.1 are explained in figure 4.