Figure 1

Expression of different NS4B proteins in Huh7 cells. Huh7 cells were transfected with NS4B full-length (FL, aa 1–261), deltaCTD (aa 1–192), CTD (aa 188–261) or CTD substitution-Cysteines (CTD sub-Cys) and 24 h later processed for indirect immunofluorescence. Cells were double labeled with antibodies reacting against Myc-epitope-tag at the C-terminal end of each protein (in red) and A. protein disulphide isomerase (PDI) or B. Cytochrome C oxidase subunit IV (COX-IV) (in green), in first and second panels respectively. Third panels show merged images.