Figure 4
From: Transduction of rat pancreatic islets with pseudotyped adeno-associated virus vectors
Transduction of rat islets with AAVTGFP2/5. (A) In vitro expression of TGF-beta 1 in conditioned medium taken on day 6 after transduction of rat islets with AAVTGFP2/5 (plus infection with Ad5) as measured by ELISA. As a negative control, islets were infected with Ad5 only. The error bars indicate the standard deviation. (B) Insulin secretion in vitro from rat islets transduced with AAVTGFP2/5 and infected with Ad5. Insulin levels in conditioned medium on day 6 after transduction were measured by radioimmunoassay. As a negative control, rat islets were infected with Ad5 only. The error bars indicate the standard deviation. (C) Blood glucose levels of NOD-SCID mice transplanted with transduced rat islets. Islets were isolated from Wistar rats and transduced with AAVTGFP2/5. Transduced islets were then transplanted into diabetic NOD-SCID mice (TGF). Other NOD-SCID mice were either transplanted with mock-transduced islets or sham operated. The error bars indicate the standard error (n = 6).