Transduction of rat islets with a high MOI of pseudotyped AAV2/8 vectors and a high Ad5 MOI. Rat islets were transduced with AAV2-nls-eGFP packaged into an AAV8 capsid at an AAV MOI of 1500 (B) or 10,000 (C). All islets were infected with Ad5 at an MOI of 10. (A) Ad5 only. The islets were visualized by fluoresence confocal microscopy on day 5 after transduction.