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Figure 8 | Virology Journal

Figure 8

From: The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment

Figure 8

Analysis of IRF-3 and N TAIL interaction in yeast. (A) Yeast growth and X-gal expression after co-transformation with bait-LexA and BD-prey encoding plasmid in glucose -His-Trp + X-Gal and in galactose/raffinose -His-Ura-Trp + X-Gal medium. (B) IRF-3-LexA and PCT-LexA expression level in yeast detected by western blot using anti-HA mAb. Ctr+ is the anti-LexA peptide aptamer RG22C, Ctr- is the irrelevant peptide aptamer C5C. Ø indicates LexA alone.

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