Figure 4
Analysis of N TAIL structural transitions in the presence of IRF-3 RD or XD by far-UV CD. Far-UV CD spectra of NTAIL alone (black line) or in the presence of a two-fold molar excess of IRF-3 RD (A) or XD (B). Spectra were recorded in 10 mM sodium phosphate buffer at pH 7. In the mixture containing NTAIL + IRF-3 RD, the concentration of NTAIL is 1.4 μM, while that of IRF-3 RD is 2.8 μM. In the mixture containing NTAIL + XD, the concentration of NTAIL is 3.5 μM, while that of XD is 7 μM. The CD spectra of XD or IRF-3 RD alone (grey lines), as well as the theoretical average curves calculated by assuming that no structural variations occur (see Materials and Methods) are also shown. Data are representative of one out of three independent experiments.