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Figure 3 | Virology Journal

Figure 3

From: The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environment

Figure 3

Purification of IRF-3 RD from E. coli. (A) Coomassie blue staining of a 15% SDS-PAGE. TF: bacterial lysate (total fraction); SN: clarified supernatant (soluble fraction); IMAC: eluent from Immobilized Metal Affinity Chromatography; HITRAP: eluent from Ion Exchange Chromatography. GF: eluent from Gel Filtration. (B) Elution profile of IRF-3 RD from analytical SEC-MALS in buffer C. The peak containing IRF-3 RD is highlighted and the inferred RS is also shown.

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Virology Journal

ISSN: 1743-422X