Figure 2From: The interaction between the measles virus nucleoprotein and the Interferon Regulator Factor 3 relies on a specific cellular environmentReciprocal coimmunoprecipitation of myc-IRF-3 and flag-N proteins. Flag-N was co-expressed with myc-IRF3 in 293T cells and immunoprecipitated by either anti-flag mAb or rabbit polyclonal anti-IRF3 antibodies. After elution by Flagx3 peptide or Laemli buffer, immunoprecipitated proteins were analysed by Western Blotting using either anti-flag or anti-myc mAb. Note that the western blot was overexposed so as to reveal flag-N co-immunoprecipitated with myc-IRF-3Back to article page