Replication of HSV-1 in DCs isolated from STAT1-/- mice. Subconfluent monolayers of DC cells from STAT1-/- and parental Wt STAT1+/+ 129SVE mice were infected with 10 PFU/cell as in Fig. 1. Panel A. Virus replication was determined as in Fig. 1. Each point represents the mean ± SEM (n = 16). Panel B. DNA was isolated and the amount of viral genomic DNA was determined by Taq-Man PCR as described in Materials and Methods and normalized to GAPDH DNA. Each point represents the mean ± SEM (n = 6). Note that the DNA levels are normalized to the levels present one hour after virus is first added to the cell monolayer (the adsorption period), a time is routinely taken as t = 0. However, significant levels of ICP0 and ICP4 DNA are already present at this time (Ct of 20–21) which masks these DNA levels at early times.